FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005482864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48288432
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA5096201.0553666352222828No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC935060.19364058041892931No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG596900.12361138584909943No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG527340.10920627946668468No Hit
CAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAG502380.10403733962618625No Hit
CTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATT483270.10007987006080463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)