FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005482865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48288432
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA2023980.4191438645181107No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1254800.2598551967891606No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1070870.22176532880587219No Hit
CGGGAAGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG1031360.21358324494777547No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG899640.18630549030873483No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG849090.17583714459810995No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC818010.169400820469797No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT695030.14393302313067446No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA625190.12946993184620284No Hit
CCGGGAAGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA591210.12243304980372939No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA512630.10616000121933965No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCTG507630.10512455653975263No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA485080.10045470103481513No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG483120.10004880672041701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)