FastQCFastQC Report
Thu 12 May 2022
EGAF00005482890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39724215
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4022351.0125687820388647No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC2394410.602758292391681No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG950400.23924953583097866No Hit
TGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGG754300.1898841802160219No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTAT635030.1598596724944722No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA518000.13039905256781034No Hit
TGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT510990.12863438585255868No Hit
TTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC497130.12514533012169027No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA453820.11424266030178318No Hit
GATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGG435850.10971897116154467No Hit
GGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAA405460.10206872558715131No Hit
GCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCA405330.102035999956198No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)