FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13680426
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2959572.163360994752649Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1504500.0132.080721
CTGCAAT1498700.0131.986012
TGCAATT1705300.0126.184993
ACCGCAA186650.0125.451331
CCGCAAT188900.0123.745182
CGCAATT204000.0121.938133
GCAATTT2221350.0108.7081454
AATTTCC2314650.0107.010286
CAATTTC2288600.0106.393965
TTTCCAC2324400.0106.025248
ATTTCCA2376400.0103.946097
TTCCACA2384650.0103.543939
CGGCAAT34250.068.12262
ACGGCAA43500.053.477761
ATGCAAT219650.050.5386282
ATTGCAA115250.041.693951
GTTCCAC92850.039.40995
ACGCAAT26850.037.511672
GGCAATT68050.035.2448583
CATGCAA111850.035.032031
Produced by FastQC (version 0.11.5)