FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13282770
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1518961.1435566527162633Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1310550.0129.828751
CTGCAAT1307500.0129.608142
TGCAATT1482100.0124.230043
CCGCAAT157900.0122.48022
CGCAATT167100.0122.371423
ACCGCAA159450.0121.487861
GCAATTT1904800.0107.724044
AATTTCC1989200.0105.870056
CAATTTC1970150.0104.9893955
TTTCCAC2013750.0103.935758
TTCCACA2054350.0101.920489
ATTTCCA2062850.0101.7604457
CGGCAAT34700.045.9401782
ATGCAAT193500.045.348472
CCGATCT43000.044.318346
ATTGCAA112700.043.9866261
TCCGATC41700.040.4873475
ACGGCAA42050.038.029171
TTCCGAT44550.037.7345964
ACGCAAT23100.036.3869322
Produced by FastQC (version 0.11.5)