FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9861760
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT146213114.826268333441494Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT356410.3614060776169771Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT163640.1659338698163411Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT139930.14189150820948798Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC119900.12158073203971706RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC118590.12025236874553832RNA PCR Primer, Index 16 (96% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT113830.115425644104095Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA103190.10463649490557467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1309400.0135.643422
ACTGCAA1320750.0135.046981
TGCAATT1507500.0128.36463
CGCAATT178850.0125.84113
CCGCAAT169900.0125.6888662
ACCGCAA181400.0117.84851
GCAATTT2028450.0107.254184
AATTTCC2089100.0106.707716
CAATTTC2061900.0106.308455
TTTCCAC2103300.0105.360518
ATTTCCA2119050.0104.847547
TTCCACA2160250.0102.774129
CGGCAAT30650.078.254182
AGATTGG87650.074.96921
GATTGGA89450.072.6644742
ATTGGAA95700.067.464583
GACCGGA40350.062.8542332
ATTGCAA83550.060.726091
GGCAATT41950.058.9023253
AGACCGG43800.058.748331
Produced by FastQC (version 0.11.5)