FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13013400
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134882910.36492384772619Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT302040.23209922080317213Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT141130.10844975179430433Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1486000.0133.754972
ACTGCAA1501700.0132.542531
TGCAATT1700050.0127.970483
CCGCAAT196400.0125.515042
CGCAATT211000.0123.732773
ACCGCAA202050.0121.7254261
GCAATTT2284350.0107.5333944
AATTTCC2365950.0106.305826
CAATTTC2328050.0106.3031545
TTTCCAC2380500.0104.9387058
ATTTCCA2413250.0103.823857
TTCCACA2448650.0102.10399
CGGCAAT38050.068.747282
ATGCAAT193850.058.7236822
ACGGCAA47100.055.550941
GATTGGA98300.055.1382
AGATTGG99200.055.0954781
ATTGCAA100600.054.978961
GGCAATT51250.053.4442333
ATTGGAA104300.052.0355833
Produced by FastQC (version 0.11.5)