FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12944094
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5406564.176854710727533Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT132310.10221650120896834Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1156000.0128.802492
ACTGCAA1169500.0127.8246461
TGCAATT1307500.0124.258413
ACCGCAA138700.0118.398711
CGCAATT146850.0117.538553
CCGCAAT140650.0116.48592
GCAATTT1701450.0106.351844
AATTTCC1780200.0104.334496
CAATTTC1759350.0103.605935
TTTCCAC1802300.0102.543338
ATTTCCA1842950.0100.5842747
TTCCACA1849100.0100.07349
CGGCAAT30550.057.400632
ATGCAAT185150.046.2992062
ACGGCAA40650.043.4563181
TCCGATC45850.038.4042635
TTCCGAT45600.037.9790344
CCGATCT49800.037.2498746
ATTGCAA108200.034.7352521
GACCGGA31900.032.483132
Produced by FastQC (version 0.11.5)