FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005482926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41155434
Sequences flagged as poor quality0
Sequence length25-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT10516712.5553636489412312No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1853160.4502831873914876No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA998700.2426654035527848No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC870240.21145202842472757No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA820330.19932483277906873No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC542780.13188537873273307No Hit
CAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAA536390.13033272835854434No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA505980.12294366765759292No Hit
GGCAGTTGCGGCGAAGCAACGCCAAAAAAGCCAGCTTCACGCTCAGGAGA485560.11798198993600699No Hit
AAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAA439050.10668093063968176No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG435270.10576246140424615No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)