FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9804234
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1471981.5013717542849345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT653850.0124.554282
ACTGCAA666400.0123.175461
TGCAATT718850.0120.95283
ACCGCAA75150.0116.279261
CGCAATT81700.0112.373453
CCGCAAT80100.0108.918862
GCAATTT919350.0105.0246054
AATTTCC982300.0101.045686
CAATTTC972050.0100.284825
TTTCCAC990700.099.615888
TTCCACA1021900.096.801389
ATTTCCA1029100.096.17347
CGGCAAT21350.041.7461432
TCCGATC29300.037.8380285
CCGATCT35350.034.6419226
TTCCGAT33550.031.9649414
ACGGCAA29000.030.8342861
ATGCAAT109800.029.8294492
CGATCCG910900.028.32197220-24
GTAGTCG901700.028.21801815-19
Produced by FastQC (version 0.11.5)