FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11177797
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5993205.3617005211313105Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT142960.12789640033720417Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT906100.0130.512772
ACTGCAA931150.0127.448031
TGCAATT998150.0127.3117453
CGCAATT115250.0122.6663063
CCGCAAT112800.0118.392782
GCAATTT1264000.0112.103144
ACCGCAA121850.0110.242591
AATTTCC1320100.0110.046226
CAATTTC1305950.0109.451925
TTTCCAC1341550.0107.7323768
ATTTCCA1375100.0105.314687
TTCCACA1375700.0105.110739
CGGCAAT23150.051.0206032
ATGCAAT127750.043.95922
TCCGATC43450.043.193755
TTCCGAT48350.039.1156964
CCGATCT51850.038.711676
ATTGCAA81450.038.4644171
ACGGCAA32950.036.17941
AGACCGG32500.035.5620461
Produced by FastQC (version 0.11.5)