FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14220696
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3545672.493316782807255Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT595900.0114.6314542
TGCAATT644850.0114.2676243
ACTGCAA632400.0108.304591
CGCAATT76900.0102.142533
GCAATTT806650.0101.929434
AATTTCC875250.096.557246
CAATTTC867150.095.678965
CCGCAAT80450.094.482882
TTTCCAC902450.093.484118
ACCGCAA82450.092.565291
TTCCACA927550.091.079379
ATTTCCA950700.088.7929467
TCCGATC52550.043.986885
TTCCGAT56600.040.4551964
CCGATCT63650.038.9344836
CGGCAAT31100.028.6581672
CTTCCGA81100.028.5912273
ATGCAAT132350.028.360212
CGATCCG761100.028.0359120-24
GTAGTCG759000.027.78016515-19
Produced by FastQC (version 0.11.5)