FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9653092
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2673892.769982923606239Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT572950.0122.130062
ACTGCAA590150.0118.857641
TGCAATT633650.0118.595513
CCGCAAT63850.0109.3989642
ACCGCAA64650.0108.13811
CGCAATT66900.0107.6607363
GCAATTT788100.0104.906264
AATTTCC840300.0100.994626
CAATTTC829850.0100.484095
TTTCCAC847600.099.4388358
TTCCACA872350.096.592679
ATTTCCA885200.095.7307367
TCCGATC32250.046.509075
CCGATCT37400.043.5925676
TTCCGAT40000.036.7733424
CGGCAAT18950.036.707862
CGGAAGT42750.036.6112635
ATGCAAT104350.031.2476312
CTTCCGA49200.031.0752353
CGATCCG768400.028.1935420-24
Produced by FastQC (version 0.11.5)