FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14139268
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8939566.3225055215022445Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT212940.15060185576792234Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT639100.0114.998232
TGCAATT701150.0112.819963
ACTGCAA669800.0110.026231
GCAATTT854250.0102.07594
CGCAATT78100.096.952463
CAATTTC914050.096.207385
AATTTCC940900.095.502446
TTTCCAC953450.093.7948
TTCCACA979950.091.38339
CCGCAAT81050.089.490032
ACCGCAA81750.088.662381
ATTTCCA1014050.088.460367
GACCGGA35700.045.2616352
AGACCGG39400.040.2246441
TCCGATC49150.032.4341055
CGGCAAT27800.031.2773552
CGGAAGA5126650.029.9177135
AGAGCAC4989700.029.8301689
TTCCGAT53150.029.7201794
TCGGAAG5151050.029.7123814
Produced by FastQC (version 0.11.5)