FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5057817
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT115768822.889084361889726Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134580.2660831738277601Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT106480.21052560818234428Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC94900.18763035515124413RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC94170.18618704472700376RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC86700.17141782709813344Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC73090.14450898480510466Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71520.1414048788242042Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT58590.11584049007704311Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC56740.11218278557725596Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA564300.0134.983051
CTGCAAT563350.0134.798452
TGCAATT629800.0128.79073
CCGCAAT76450.0125.490942
ACCGCAA78850.0121.963771
CGCAATT82400.0121.881533
GCAATTT833800.0110.30734
AATTTCC854750.0108.476836
CAATTTC852000.0108.282655
TTTCCAC858150.0107.2765358
ATTTCCA871700.0106.116067
TTCCACA873200.0105.850789
CGGCAAT15050.073.182792
GACCGGA18500.060.7102242
AGACCGG19250.058.5736161
CGGAAGA3491200.055.27325
AGATTGG40950.055.2468571
GATTGGA40700.055.1911132
TCGGAAG3501450.055.047244
ATCGGAA3542950.055.0405583
Produced by FastQC (version 0.11.5)