FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15163288
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7238594.773760150173234Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1243050.0129.38622
ACTGCAA1258300.0128.070541
TGCAATT1382550.0125.828323
ACCGCAA172700.0123.984671
CGCAATT185200.0121.4121553
CCGCAAT176950.0121.134822
GCAATTT1807400.0109.4374854
CAATTTC1877800.0105.728215
AATTTCC1891150.0105.556626
TTTCCAC1889350.0104.8043448
TTCCACA1918300.0103.33989
ATTTCCA1948950.0102.008357
CGGCAAT38350.055.930372
ACGGCAA47550.044.9696351
TCCGATC81300.043.1396065
ATGCAAT188300.042.985722
CCGATCT83000.042.3433346
TTCCGAT84000.041.752984
CGATCTA37800.037.5733537
ATTGCAA130800.036.143331
Produced by FastQC (version 0.11.5)