FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7281874
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT102309014.049817395906603Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT127290.1748039035006648Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA103220.1417492255427655No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92210.1266294912545864Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA83840.11513519734068454No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC82980.11395418267330636RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC81970.11256717707557148RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC79040.10854348756927132Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1299350.0139.309421
CTGCAAT1304900.0138.274372
CCGCAAT165650.0131.716112
TGCAATT1472900.0131.087743
ACCGCAA168550.0130.036481
CGCAATT177400.0128.17963
GCAATTT1985350.0109.092244
AATTTCC2011900.0108.477416
CAATTTC2007050.0108.291855
TTTCCAC2011650.0107.5744558
ATTTCCA2033550.0106.900567
TTCCACA2037750.0106.338869
CGGCAAT22200.092.048282
ATTGCAA68850.070.8360061
ACGGCAA29700.069.115561
GGCAATT30850.068.587863
ATGCAAT140400.055.431512
GCAATTC47150.053.0221064
TATGCAA36900.052.867851
AGATTGG47850.051.1140821
Produced by FastQC (version 0.11.5)