FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11930454
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2328771.9519542173332214Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA921500.0129.953191
CTGCAAT920500.0129.550452
TGCAATT1025600.0123.3752443
CGCAATT123400.0121.024923
ACCGCAA118150.0119.991751
CCGCAAT121850.0116.379732
GCAATTT1350450.0104.9977344
AATTTCC1402000.0102.258656
CAATTTC1392050.0102.193145
TTTCCAC1413000.0100.4719858
TTCCACA1440100.098.717159
ATTTCCA1453350.098.245947
CGGCAAT27250.048.9286582
ACGGCAA35000.038.8639451
ATTGCAA84850.035.2341081
ATGCAAT133500.033.3272252
GGCAATT45400.031.2832763
CGATCCG1345950.028.36926320-24
GTAGTCG1328850.028.29873715-19
CCGATGT1350750.028.09989225-29
Produced by FastQC (version 0.11.5)