FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10872401
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2570212.3639764574540623Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT954750.0132.692342
ACTGCAA960950.0132.300231
TGCAATT1049400.0128.885743
CGCAATT129050.0126.277553
CCGCAAT130450.0120.367582
ACCGCAA131800.0119.595031
GCAATTT1402050.0108.612864
AATTTCC1437850.0106.891276
CAATTTC1435700.0106.486095
TTTCCAC1450100.0105.13648
TTCCACA1477200.0103.330259
ATTTCCA1486400.0103.012437
CGGCAAT26050.059.2470322
ATTGCAA78550.049.4498181
ATGCAAT101250.045.2289732
GGCAATT38000.042.1408583
ACGGCAA37750.041.2350461
TATGCAA50000.034.478471
TCCGATC34700.033.8283045
GCAATTC54550.030.8168284
Produced by FastQC (version 0.11.5)