FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005482990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005482990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9424456
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5474065.808356471715715Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1053800.0134.089661
CTGCAAT1052800.0133.845542
ACCGCAA138550.0127.5565951
CCGCAAT139100.0127.364652
TGCAATT1164250.0127.306463
CGCAATT148800.0123.444633
GCAATTT1515200.0110.444774
CAATTTC1557300.0107.8870855
AATTTCC1570850.0107.593026
TTTCCAC1566450.0107.0666668
TTCCACA1587600.0105.745299
ATTTCCA1609700.0104.5950857
CGGCAAT24150.076.210312
ACGGCAA32550.056.7759781
TCCGATC39850.042.7303775
GGCAATT44300.042.3636323
TTCCGAT41350.040.3041234
ATTGCAA76850.039.290371
CCGATCT45600.036.22996
ATGCAAT132300.035.5452882
Produced by FastQC (version 0.11.5)