FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15228026
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT14915649.794861133018816Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181270.1190370964693651Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1403250.0131.869382
ACTGCAA1414800.0131.238431
TGCAATT1565650.0126.133013
CGCAATT200450.0122.149383
CCGCAAT193800.0121.068792
ACCGCAA196350.0119.8399351
GCAATTT2063400.0108.208084
CAATTTC2108100.0106.205825
AATTTCC2117800.0106.1812746
TTTCCAC2135700.0104.468218
TTCCACA2162200.0103.412379
ATTTCCA2185000.0102.6052257
CGGCAAT38300.059.4073262
ACGGCAA52350.043.9200551
ATGCAAT199600.041.894252
TTCCGAT82000.041.6213534
TCCGATC85300.040.09615
CCGATCT88950.038.7766346
GACCGGA43650.038.6794972
AGACCGG47700.035.388491
Produced by FastQC (version 0.11.5)