FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022440
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT58364014.509601137617963Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT197680.49144300474338964Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT177720.44182138204671795Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT138260.34372172114438004Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT137730.34240411292648243Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT64570.16052445779178806Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT41130.10225136981533596Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT215650.0121.139592
TGCAATT240050.0118.724663
ACTGCAA231600.0111.645031
CGCAATT25700.0108.5817263
GCAATTT295550.0106.976724
AATTTCC331800.0105.749186
CAATTTC318550.0105.320225
TTTCCAC347650.0100.341648
ATTTCCA350900.099.805977
GACCGGA33300.094.905862
TTCCACA371150.094.353089
CCGCAAT27450.093.742552
AGACCGG33600.093.011061
ACCGGAA39200.081.726973
AGATTGG46450.079.144161
GATTGGA46200.079.075032
ACCGCAA32650.078.839131
ATTGGAA48400.075.62673
ACGGAAG33750.074.9525154
GAACGGA35600.070.856552
Produced by FastQC (version 0.11.5)