FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3354608
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1064073.171965248994815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT35010.1043639077948899Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA219500.0119.097251
CTGCAAT230850.0114.412162
CCGCAAT28150.0103.764882
ACCGCAA28400.0102.134541
CGCAATT30900.098.980983
TGCAATT292900.098.334593
GCAATTT356050.090.663094
AATTTCC395350.089.952836
CAATTTC384100.088.779595
TTTCCAC403250.087.578098
ATTTCCA412250.086.158477
TTCCACA423400.083.544319
GACCGGA10750.076.189252
AGACCGG11900.067.031161
ACCGGAA17100.047.8938943
CGGCAAT8250.047.442062
CCGATCT13600.043.7062956
GTTCCAC27200.042.1003765
ACGGCAA9500.041.2193451
TCCGATC12500.038.8474585
Produced by FastQC (version 0.11.5)