FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9278410
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4560094.914732157772722Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT160130.17258344910388743Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143070.15419667809463045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT112470.12121688953171934Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110370.11895357070877445Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT553750.0114.613512
ACTGCAA554550.0113.085291
TGCAATT685450.0102.8543
CCGCAAT72150.0100.76472
ACCGCAA72300.098.793661
CGCAATT79650.097.094963
GCAATTT838550.094.325384
AATTTCC942950.093.300776
CAATTTC912100.091.901715
TTTCCAC977200.089.70388
ATTTCCA986550.089.104397
TTCCACA1027700.085.43329
GACCGGA37950.073.343772
AGACCGG38300.072.894221
ACCGGAA51800.054.290023
CGGCAAT24300.051.305742
GTTCCAC72250.047.7517745
AGAACGG47500.046.2572861
ACGGAAG49050.045.8074464
GAACGGA49600.044.133572
Produced by FastQC (version 0.11.5)