FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370168
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT63303714.48541566365412Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT219280.5017656071803189Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT193420.44259168068595994Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT150950.3454100620388049Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT150110.34348793913643594Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT74120.16960446371855728Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT45960.10516758165818797Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT375650.0132.570312
TGCAATT421050.0128.668563
ACTGCAA384400.0127.9442441
CGCAATT51150.0119.746853
CCGCAAT48500.0118.075092
GCAATTT536150.0113.84544
AATTTCC603650.0113.243776
CAATTTC578300.0112.728275
ATTTCCA623350.0109.396167
TTTCCAC622250.0109.240058
ACCGCAA53650.0105.281011
TTCCACA657950.0103.3335349
GACCGGA34800.098.735212
AGACCGG34950.097.506371
AGATTGG48050.082.240441
ACCGGAA42050.082.225293
GATTGGA48300.081.794162
ATTGGAA51850.077.029293
GAACGGA37400.075.590282
AGAACGG37650.074.529521
Produced by FastQC (version 0.11.5)