FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062563
Sequences flagged as poor quality0
Sequence length6
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG124720.30699831608765205No Hit
GGGGTG80710.19866768835338677No Hit
GGGGGT80530.1982246183013039No Hit
TGGGGG75050.1847355967156694No Hit
GTGGGG74490.18335715655363377No Hit
GGGTGG72450.17833569596336107No Hit
GGTGGG68990.16981890496221227No Hit
AGGGGG60000.14769001736096155No Hit
GGGGCG59680.14690233726836976No Hit
CGGGGG59130.1455485121092276No Hit
GGGCGG55290.13609635099812606No Hit
GGGTTG53990.1328964006219719No Hit
GAGGGG53460.13159180546861673No Hit
GGGGAG53390.1314195004483623No Hit
GCGGGG52750.1298441402631787No Hit
GGGGGC52500.12922876519084137No Hit
TGGGTG52180.12844108509824956No Hit
GGCGGG51410.12654572987545054No Hit
GGGGGA51310.1262995798465156No Hit
GGAGGG51120.12583189479153922No Hit
GGGAGG49060.12076120419547956No Hit
TTGGGG48520.11943199403923091No Hit
TGTGGG48330.11896430898425453No Hit
GTTGGG48210.1186689289495326No Hit
GTGTGG47990.11812739888587573No Hit
GGGGTT47720.1174627938077514No Hit
GGTGGT46780.11514898353576301No Hit
TGGTGG46350.11409053841134278No Hit
GGGTGT45890.1129582482782421No Hit
GTGGGT45240.11135827309016501No Hit
GTGGTG45070.11093981804097561No Hit
GGTTGG44410.10931522785000503No Hit
ATGGGG43000.10584451244202245No Hit
TAGGGG42260.10402300222790391No Hit
GGGGCT42210.10389992721343644No Hit
GGTGTG41950.10325993713820561No Hit
TGGGGT41550.10227533702246586No Hit
GTAGGG41300.10165996195012852No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)