FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296298
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT30451813.261257902937684Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105690.46026256174068003Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT93320.4063932468695265Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT73040.3180771833620898Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72080.31389654130256617Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT35120.15294182201090625Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA208400.0126.014381
CTGCAAT213950.0124.228992
TGCAATT261150.0112.4818653
CCGCAAT27200.0111.3862842
CGCAATT29550.0111.105783
ACCGCAA27300.0109.452041
AATTTCC363900.0101.100766
GCAATTT329900.0100.199214
CAATTTC352550.099.928965
GACCGGA17450.099.687372
AGACCGG17350.098.651081
ATTTCCA375450.097.8940667
TTTCCAC374600.097.881898
TTCCACA393850.093.353469
CGGCAAT7400.086.193652
ACCGGAA21000.083.5202943
GATTGGA23500.079.266482
ATTGGAA24850.075.830333
AGATTGG24850.075.589711
AGAACGG17150.074.005371
Produced by FastQC (version 0.11.5)