FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5137537
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT173435733.75853059549742Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT597741.1634758056243681Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT538931.0490046105750672Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT418250.8141060589928597Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT416000.8097265284902084Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT194740.37905323114947886Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT127530.2482317888902795Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT112740.21944367505285117Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC108840.2118524888482555RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC107240.20873815604637008RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98960.19262148379661304Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCTTATGCCGTCTT97370.18952661557473943Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC86190.16776521512156506Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC85140.16572143422032776Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACGCCAGTCCATCTCGTATGCCGTCTT75960.1478529497695102Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT70960.13812065976361826Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT62200.12106968767329558Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC58290.11345903688868811RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTAGTATGCCGTCTT57730.11236902040802821Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC57240.1114152559874508Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC53800.10471944046339715TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAATCTCGTATGCCGTCTT52650.102481013762042TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT247200.0120.863592
TGCAATT283450.0116.987083
AGACCGG77850.0116.049541
GACCGGA78900.0115.0173342
ACTGCAA261250.0113.2923661
AGATTGG92850.0111.5923161
GATTGGA94550.0109.471892
ACCGGAA85500.0106.898653
GCAATTT353750.0105.764664
AATTTCC401750.0104.2587666
CAATTTC385750.0103.699765
CCGGAAG89300.0102.511114
TTTCCAC414150.0100.6801158
CGCAATT37100.0100.613573
ATTTCCA418000.099.9618767
ATTGGAA104700.099.4102253
TTGGAAG105450.098.289814
ACGGAAG74150.096.868274
TGATCGG23150.096.154891
AGAACGG75150.096.0981
Produced by FastQC (version 0.11.5)