FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5137537
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGG177880.34623594924961126No Hit
GGGGGT103890.2022175217424225No Hit
GGGGTG100400.19542438331830994No Hit
GTGGGG93720.1824220438704383No Hit
TGGGGG92220.17950235686867072No Hit
GGGTGG91600.17829555290794014No Hit
GGTGGG84390.16426159071944396No Hit
AGGGGG83720.16295746385865445No Hit
CGGGGG78370.15254391355235009No Hit
GCGGGG76330.14857313922994617No Hit
GGCGGG75520.1469965082489917No Hit
GGGGCG73370.14281162354645816No Hit
GGGGGA73260.14259751316632854No Hit
GGGGGC72870.14183839454586897No Hit
GAGGGG72310.14074837806520907No Hit
GGGGAG71920.1399892594447495No Hit
GGGCGG71880.13991140112470235No Hit
GGAGGG71350.1388797783840778No Hit
GGGAGG64630.12579958061615906No Hit
GGGGTT60790.11832518189163407No Hit
GGGTTG59120.11507459702966617No Hit
GGGTGT58010.11291402864835814No Hit
GTGTGG57720.11234955582801642No Hit
GGTGGT57510.11194079964776896No Hit
TGGTGG56870.1106950665270148No Hit
TTGGGG56860.11067560194700302No Hit
GTGGGT55780.10857342730573034No Hit
GTGGTG54690.10645178808444591No Hit
GGTTGG54620.10631553602436343No Hit
GGGTAG53960.10503087374358569No Hit
GGGGTA53730.10458318840331467No Hit
TGTGGG51630.10049562660084006No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)