FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992416
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2479268.28514484617112Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86430.28883016265118217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT76360.2551784243901917Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT60560.2023782789558671Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT59150.19766636724305714Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA213900.0117.5111161
CTGCAAT217050.0116.978842
CCGCAAT26050.0103.226972
TGCAATT292700.0100.826753
ACCGCAA26800.099.010721
CGCAATT30650.096.003333
AATTTCC411000.089.4527056
CAATTTC395850.088.709145
GCAATTT370650.088.6169054
GACCGGA14350.086.876742
AGACCGG14250.085.982041
ATTTCCA428050.085.804967
TTTCCAC427550.085.564818
TTCCACA450250.081.357969
CGGCAAT7200.071.4747852
ACCGGAA19200.066.813273
GTTCCAC27350.064.3952945
AGATTGG28200.063.7585561
GATTGGA28650.062.488392
AGAACGG19700.059.6190031
Produced by FastQC (version 0.11.5)