FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4541098
Sequences flagged as poor quality0
Sequence length6
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTC1003092.2089151126005206No Hit
GGGGGG301180.6632316677596476No Hit
GTGGGG136710.3010505388784827No Hit
CGGGGG130120.28653863008461833No Hit
GGGTGG127640.281077395819249No Hit
GGGGGT126210.2779283776742982No Hit
AGGGGG123580.27213682682029766No Hit
TGGGGG119840.2639009332104262No Hit
GGGGTG117500.25874799442777935No Hit
GGTGGG115350.2540134566573987No Hit
GGAGGG113480.249895509852463No Hit
GGGGCG111670.24590968968297974No Hit
GGCGGG111660.24588766857707103No Hit
GAGGGG108880.2397658011344393No Hit
GGGGGA107820.2374315639081121No Hit
GGGCGG107710.23718933174311585No Hit
GCGGGG106060.23355584926817258No Hit
GGGGGC104040.22910758587460564No Hit
GGGAGG102690.22613473657692476No Hit
GGGGAG102280.22523187123466615No Hit
GTGTGG73200.16119449525202934No Hit
GTAGGG67330.14826810608359475No Hit
ATGGGG66820.14714502968224866No Hit
TTGGGG66290.14597791106908506No Hit
CTGGGG63250.1392834948728259No Hit
GGGGTT62820.1383365873187498No Hit
ATTTTC62270.1371254264937687No Hit
GGTGGT60370.1329414163711067No Hit
GTTGGG60050.1322367409820268No Hit
GGGTGT60030.13219269877020934No Hit
GTGGGT59910.13192844549930435No Hit
GTGGTG59250.13047505250932703No Hit
GGGGTA58210.12818485749481734No Hit
AGGTGG58200.1281628363889086No Hit
GAAGGG57080.1256964725271289No Hit
GGTTGG56950.12541019815031518No Hit
GGGTAG56760.12499179713804899No Hit
TGGGGT56750.12496977603214025No Hit
TGGTGG56330.12404488958397286No Hit
GTGAGG56230.12382467852488539No Hit
TGTGGG56040.12340627751261919No Hit
GTCGGG55850.12298787650035299No Hit
AGGGTG55660.12256947548808679No Hit
AGCGGG55550.12232724332309058No Hit
AGTGGG54990.12109406139220073No Hit
AAGGGG54710.12047747042675581No Hit
CGTGGG54700.12045544932084706No Hit
AGGGGT54600.12023523826175961No Hit
GTGGCG54390.1197727950376759No Hit
GGATGG54380.11975077393176715No Hit
TAGGGG53760.11838546536542484No Hit
CAGGGG53260.11728441006998748No Hit
GGAGTG53120.11697611458726502No Hit
CGGTGG52800.11627143919818511No Hit
CGGGGT52790.11624941809227636No Hit
GGGTTG52520.11565484823274018No Hit
GGGGCT52320.11521442611456525No Hit
TGAGGG52220.11499421505547777No Hit
GTGGGA52210.11497219394956902No Hit
GGCTGG51840.11415741303094538No Hit
CGGGTG51370.11312242105323425No Hit
GGGCGT51090.11250583008778933No Hit
CGAGGG50740.11173509138098318No Hit
GAGGGT50730.11171307027507443No Hit
TGGGTG50440.11107445820372078No Hit
TGGGGA50420.11103041599190328No Hit
GTGGAG50080.11028169839100588No Hit
CGCGGG50020.1101495717555534No Hit
GGGGCA50020.1101495717555534No Hit
GTGCGG50000.1101055295437359No Hit
GTGGGC49530.10907053756602479No Hit
CCGGGG49300.1085640521301236No Hit
GGGTGA49040.10799150337649616No Hit
GGGTCG48990.10788139784695243No Hit
CGGCGG48910.10770522899968245No Hit
GGAGGT48900.1076832078937737No Hit
GGGGAA48860.10759512347013872No Hit
GGGAGT48810.10748501794059498No Hit
GGTAGG47900.10548109730289898No Hit
CGGGGA47860.105393012879264No Hit
AGAGGG47820.10530492845562901No Hit
GCGGGT47790.10523886513790277No Hit
GCCGGG47740.10512875960835905No Hit
GGCCGG47600.10482046412563657No Hit
TGGAGG47560.1047323797020016No Hit
GGGCCG47290.10413780984246541No Hit
CATTTC47160.1038515354656517No Hit
GCAGGG46830.10312483897066305No Hit
GACGGG46720.10288260680566683No Hit
GGGCAG46530.10246420579340063No Hit
AGGGAG46510.10242016358158314No Hit
GGAAGG46240.10182559372204697No Hit
GGCAGG46170.10167144598068573No Hit
ACGGGG46120.10156134045114198No Hit
AGGGCG46030.10136315049796327No Hit
GGGGAT45980.10125304496841953No Hit
GGCGGT45890.10105485501524081No Hit
GCTGGG45750.10074655953251833No Hit
GGTCGG45670.10057039068524837No Hit
GATGGG45670.10057039068524837No Hit
GGTGTG45660.10054836957933962No Hit
AGGGGA45650.10052634847343088No Hit
GGTGAG45510.10021805299070842No Hit
TGGCGG45510.10021805299070842No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)