FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990889
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2977689.955835873547965Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103810.3470874378821815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92860.3104762497036834Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT73120.24447580635724026Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72260.2416004071030386Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT33180.11093691541210658Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA194800.0118.059211
CTGCAAT205500.0112.968012
TGCAATT258150.098.6019443
CCGCAAT24950.091.796662
ACCGCAA24700.091.5826641
AATTTCC349350.090.659146
GCAATTT312200.090.535794
CAATTTC335650.089.549885
CGCAATT27250.089.366733
TTTCCAC358000.088.144648
ATTTCCA359750.087.816617
GACCGGA17650.085.0033342
TTCCACA377400.083.749089
AGACCGG18100.080.113751
ACCGGAA20600.074.236643
GATTGGA29550.065.7336352
AGATTGG29300.065.574341
CGGCAAT8500.058.8356442
AGAACGG20900.058.279881
ACGGAAG20650.058.2608224
Produced by FastQC (version 0.11.5)