FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391682
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT45408913.388312937356744Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT157780.46519691409748914Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT139300.4107106739370024Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110400.32550221394576495Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT109300.3222589853647836Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT53800.15862336150617892Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA344850.0127.611681
CTGCAAT354550.0125.358912
CCGCAAT42450.0113.7174452
TGCAATT459150.0111.999373
CGCAATT48750.0109.2774353
ACCGCAA44500.0106.7295761
GACCGGA24950.0103.7118152
AATTTCC639800.0100.0993656
GCAATTT579450.099.0150454
CAATTTC617900.098.88285
AGACCGG26000.098.1686551
ATTTCCA656050.097.25397
TTTCCAC663750.095.916778
ACCGGAA28850.091.196593
TTCCACA700800.090.961129
GTTCCAC35650.082.135935
GATTGGA35700.081.21242
AGATTGG35950.080.679771
ACGGAAG26250.080.62414
CGGCAAT10750.079.5617142
Produced by FastQC (version 0.11.5)