FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238107
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT39253331.704287270809388Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136121.0994203247376841Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121530.9815791365366644Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT94800.7656850336844877Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92830.7497736463811286Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46180.37298876429904687Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT29320.23681313489060316Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC27210.21977098909868048RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC26490.21395565972892489RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT26350.2128249012403613Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT22980.18560592905136633Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCTTATGCCGTCTT22950.18536362366095982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC21230.17147144794432145Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACGCCAGTCCATCTCGTATGCCGTCTT17540.141667884924324Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC16530.13351027011397237Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT15170.1225257590822118Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT14770.11929502054345868Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC14490.11703350356633149RNA PCR Primer, Index 16 (100% over 22bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC13820.11162201651392005Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC13260.10709898255966568TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT115500.0133.44712
ACTGCAA116650.0130.723181
TGCAATT129750.0128.619923
CGCAATT16850.0120.4670943
CCGCAAT16150.0117.164442
AGACCGG19550.0116.090221
GACCGGA19900.0115.487242
GATTGGA20300.0114.283042
AGATTGG20900.0113.101751
GCAATTT170350.0111.92874
CAATTTC182850.0111.374215
AATTTCC191200.0111.354766
ATTGGAA21050.0111.239933
ATTTCCA194700.0108.794527
ACCGGAA21550.0107.649743
TTTCCAC196700.0107.282928
TTGGAAG22700.0103.158384
ACGGAAG16300.0100.96394
TTCCACA209450.0100.607739
CCGGAAG23200.099.685174
Produced by FastQC (version 0.11.5)