FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025251
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT654156.380388802351815Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT23180.22609097674618217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT21610.21077765347217414Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT16080.15683964219493568Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT15150.14776869273963156Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA106600.0129.792861
CTGCAAT110100.0127.4140552
ACCGCAA12600.0116.254691
CCGCAAT12650.0115.766932
TGCAATT143250.0115.490093
CGCAATT15650.0109.788713
AATTTCC212250.098.0003746
CAATTTC203650.097.332755
GCAATTT190550.097.25164
ATTTCCA217300.095.255777
TTTCCAC218050.094.4293758
TTCCACA234950.087.378419
GTTCCAC12900.083.179615
CCAATTT7350.077.926414
AGACCGG4550.074.9059141
CGGCAAT3650.073.490732
GACCGGA4650.073.277152
TCGCAAT2150.070.8116152
TGATCGG1550.070.1760941
GCAAGTT10850.069.507751
Produced by FastQC (version 0.11.5)