FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3206140
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77404824.142676239964565Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT266020.8297204738408179Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT240010.7485948835671555Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184900.5767059454671349Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT183520.5724017042300087Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92690.2891015364269807Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT58280.18177621688385412Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC53410.16658661193834332RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51010.15910097500421067Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC50740.15825884084912076RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT45460.14179043959402896Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCTTATGCCGTCTT44320.13823476205031596Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC41170.12840986357426687Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA243850.0125.979611
CTGCAAT251850.0124.25772
TGCAATT318650.0114.952553
CGCAATT36750.0111.645783
AGACCGG36450.0109.800951
GACCGGA37400.0108.736292
CCGCAAT33700.0107.1229862
AGATTGG43850.0104.002941
AATTTCC455850.0100.375636
GCAATTT412500.099.653334
GATTGGA46050.099.4882
CAATTTC440550.099.294385
ACCGGAA41500.097.8189853
ATTTCCA468800.097.278157
TTTCCAC476950.095.3712168
ACCGCAA37150.094.4606861
ATTGGAA49400.092.741343
TTCCACA505750.090.0698559
ACGGAAG34550.089.1655354
AGAACGG34450.089.025761
Produced by FastQC (version 0.11.5)