FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148763
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT11700710.185477770436547Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT41310.3596042003441963Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT36870.3209539304451832Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT27790.24191238749855282Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT27290.23755987962704228Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT13620.11856231441994562Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA146150.0131.527921
CTGCAAT154450.0126.475862
CCGCAAT18500.0114.797572
ACCGCAA18350.0114.595871
TGCAATT209600.0110.859063
CGCAATT22250.0108.796623
GTTCCAC20000.0106.1738745
GCAAGTT14750.099.7955251
AAGTTCC16000.097.3906863
AATTTCC313500.092.4552846
CAATTTC301250.091.539325
GCACGTT5250.091.157341
GCAATTT285650.090.5547564
ATTTCCA319950.090.047767
AGTTCCA17100.089.854274
ACTTTCC17350.088.9772643
TTTCCAC327350.087.853348
CAAGTTC17700.087.225432
ACGTTCC6100.086.734533
GACCGGA7100.085.754662
Produced by FastQC (version 0.11.5)