FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654077
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4982017.487154116190721Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT173800.261193250393706Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT154750.23256418583674338Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121280.18226419682248943Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT119140.17904812342868892Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT324950.0115.310582
TGCAATT377100.0109.145293
ACTGCAA340200.0109.123761
CGCAATT43200.0100.677783
GCAATTT457650.0100.652694
AATTTCC519700.098.510736
CAATTTC501700.097.24835
TTTCCAC540650.094.075968
CCGCAAT44000.093.083982
ATTTCCA555700.091.8281567
ACCGCAA44650.090.2894441
TTCCACA569100.089.5431449
GACCGGA34950.084.6237262
AGACCGG36150.081.032031
ACCGGAA43000.068.610733
ACGGAAG37600.058.4114534
AGAACGG38650.056.280171
TGATCGG11900.053.009771
GAACGGA42550.052.3019642
CCGGAAG58350.051.1799164
Produced by FastQC (version 0.11.5)