FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510176
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4351919.649091299319583Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT149420.33129527539501785Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT135890.30129644608103984Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105190.23322814896802255Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT104030.230656187253003Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT49960.11077173041584187Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA327800.0117.8628161
CTGCAAT334700.0117.036852
TGCAATT428900.0103.397873
ACCGCAA41250.0101.764121
CCGCAAT43100.098.387872
CGCAATT47450.097.769173
AATTTCC603350.092.3741466
GCAATTT545400.091.211044
CAATTTC583600.091.0286255
ATTTCCA624250.089.1875467
TTTCCAC627300.088.523758
GACCGGA25600.087.494652
AGACCGG26600.083.675211
TTCCACA665400.083.60969
CGGCAAT12200.070.110842
ACCGGAA32850.069.066453
AGATTGG44750.067.2349551
ACGGAAG28650.066.539524
TGATCGG10950.065.5482561
GATTGGA45900.065.222942
Produced by FastQC (version 0.11.5)