FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3664269
Sequences flagged as poor quality0
Sequence length151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3037338.289047556279302Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT102630.28008314891728747Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT93470.25508498420831005Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT73570.20077674428378486Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT73090.1994667967881179Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA329850.0125.077031
CTGCAAT351450.0118.52212
CCGCAAT43450.0109.429842
ACCGCAA43650.0107.473611
TGCAATT465050.0103.301023
CGCAATT51250.0102.816373
AATTTCC648500.092.6744846
CAATTTC627900.091.5027545
GCAATTT591450.090.97914
ATTTCCA669200.089.719957
TTTCCAC677200.088.432868
TTCCACA713650.084.36039
AGACCGG20800.082.2679141
GACCGGA21150.082.2474752
GTTCCAC36800.078.9791265
ACCGGAA25000.069.581363
CGGCAAT11350.067.052522
GCAAGTT29850.063.3983541
CCAATTT28250.061.576434
GATTGGA31550.059.730442
Produced by FastQC (version 0.11.5)