FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786138
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT779902.7992152578228358Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA199600.0120.613481
CTGCAAT212500.0114.8799442
ACCGCAA23750.0112.968251
CCGCAAT25850.0105.7089842
TGCAATT267000.0101.579963
CGCAATT29700.098.8357543
AATTTCC366850.092.259966
CAATTTC353550.091.6151355
GCAATTT332900.091.483374
TTTCCAC374500.089.8148
ATTTCCA383100.088.04387
TTCCACA394250.085.478679
GACCGGA6800.067.152582
CGGCAAT6850.058.1973462
AGACCGG8100.056.399311
GTTCCAC24400.055.84395
CGTTCCA9150.044.3574754
ACGTTCC8700.043.321113
ACGGCAA9250.043.1160771
ACCGGAA11550.040.1618273
Produced by FastQC (version 0.11.5)