FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702326
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129768835.050614127443126Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT444511.2006236079696926Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT396031.0696788991569084Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT307800.8313692527346322Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT304750.8231311883394385Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144810.391132493464919Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92950.2510583886994284Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC86970.23490638047540927RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC84540.22834293900645164RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT83880.2265602758914261Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCTTATGCCGTCTT75550.20406090657602816Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT73190.19768653543745202Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC68490.1849918132546945Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACGCCAGTCCATCTCGTATGCCGTCTT56360.1522286260043011Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT49680.13418591447646697Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT49650.13410488433487489Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC47210.1275144328187199RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTAGTATGCCGTCTT42790.11557599195748834Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC41810.11292900733214742TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC40980.11068717341476682TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC40320.1089045102997413Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGCAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT38890.10504207355051932Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA59250.0118.659012
AGACCGG59650.0117.567081
AGATTGG70100.0114.9385451
GATTGGA72350.0112.301212
ACCGGAA64450.0110.0974043
CTGCAAT153750.0108.4251562
CCGGAAG66400.0106.645824
ATTGGAA77050.0105.356843
ACTGCAA156800.0105.082871
ACGGAAG51900.0102.784544
GAACGGA53700.0100.419022
AGAACGG53250.099.69221
TTGGAAG82900.098.271854
TGCAATT190550.097.8316353
GAAGAGC2546700.095.325427
AGAGCAC2552400.095.2662059
AAGAGCA2551050.095.238048
GGAAGAG2568650.095.190936
CGGAAGA2465250.094.596475
TGATCGG17750.093.972151
Produced by FastQC (version 0.11.5)