FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10032754
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT115869511.54912200578226Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT421720.4203432078569852Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT373340.3721211543709733Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT270890.2700056235805244Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT259930.25908140476682673Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT238900.2381200615503978Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT212560.21186605392696764Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT164180.1636440004409557Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT108930.10857437549051836Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT629050.0124.836612
TGCAATT710100.0122.391033
ACTGCAA650500.0119.242821
CGCAATT78100.0112.886283
GCAATTT905550.0107.536154
CCGCAAT76100.0107.373682
AATTTCC1122250.0107.336036
CAATTTC1070900.0107.287775
TTTCCAC1161400.0103.049868
ATTTCCA1177300.0102.012757
TTCCACA1221350.098.093559
ACCGCAA82450.097.895491
GACCGGA69100.097.055972
AGACCGG71400.091.7165451
ACCGGAA84450.080.5307543
ACATCGG44500.078.712781
TGATCGG27550.074.7575151
CGGCAAT27950.070.817312
AGATTGG109350.070.033091
CATCGGA50700.069.2143252
Produced by FastQC (version 0.11.5)