FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8520796
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6229817.311300493521966Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT222630.2612784063836289Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT200000.2347198548116866Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT141140.16564180154060726Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT139990.16429216237544003Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT126400.14834294824098593Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT112930.13253456601941885Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86890.10197404092293724Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT619950.0126.392182
ACTGCAA621200.0124.017131
TGCAATT725750.0122.4528353
CGCAATT78550.0115.473633
CCGCAAT72750.0112.122112
ACCGCAA73050.0110.405671
CAATTTC1072150.0109.9707955
GCAATTT907500.0109.664624
AATTTCC1134250.0108.907076
TTTCCAC1168000.0104.997918
ATTTCCA1175250.0104.676537
TTCCACA1221550.0100.674589
GACCGGA39350.091.207842
AGACCGG42500.083.79141
CGGCAAT25000.077.145992
TTCAATT164000.067.377183
TCAATTT178500.067.265334
ACCGGAA54300.066.229813
ACATCGG28350.065.492831
TGATCGG17700.062.284091
Produced by FastQC (version 0.11.5)