FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10104947
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6491496.424071298939024Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT236660.234202119021505Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT211260.20906591593206772Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT151880.15030261910329662Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT145870.14435503719118964Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT130490.1291347693362469Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT118450.11721981322613567Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT772800.0128.405722
ACTGCAA782000.0124.162141
TGCAATT917350.0124.121773
CCGCAAT88500.0116.501432
CGCAATT99800.0115.58793
ACCGCAA90000.0113.78171
CAATTTC1376100.0110.0974665
GCAATTT1165800.0109.417414
AATTTCC1456000.0109.205916
TTTCCAC1492850.0105.60768
ATTTCCA1509350.0104.8982547
TTCCACA1562850.0100.8964849
GACCGGA47150.082.886152
AGACCGG49150.078.7948761
CGGCAAT31050.076.359142
TCAATTT221650.072.81624
TTCAATT203500.072.113883
ACCGGAA58250.068.2114643
ACATCGG30200.064.1186751
CTTCAAT143250.059.6752242
Produced by FastQC (version 0.11.5)