FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579783
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT292075127.60690838365967Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1035220.9784888782690534Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT928570.8776834080623392Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT664070.6276782803579242Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT647820.612318797086859Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT597430.564690220961999Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT536080.5067022641201621Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT418100.395187689577376Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT271320.25645138468340983Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT225290.21294387607004797Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC194360.18370887191164506RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC191750.18124190259856937RNA PCR Primer, Index 16 (96% over 25bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181790.17182772085211956Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC165460.1563926216634122Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC151510.1432070960245593Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC121320.11467153910434648Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT516650.0117.0996552
GACCGGA142700.0111.935622
ACTGCAA532950.0111.86311
AGACCGG143800.0109.762591
TGCAATT638100.0107.583393
ACCGGAA156400.0103.057223
AGATTGG177450.0102.641991
GATTGGA182600.0100.93752
ACATCGG94850.099.493411
CGCAATT74200.097.3265153
CAATTTC933600.097.301845
AATTTCC981000.097.237286
GCAATTT803050.095.615224
ATTGGAA194050.095.205383
CATCGGA100850.093.751772
TTTCCAC1016650.093.2031568
ATTTCCA1021550.093.102967
TGATCGG52300.092.9239041
CCGGAAG174350.092.529824
CCGCAAT71950.091.704022
Produced by FastQC (version 0.11.5)