FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8855783
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3060783.456250000705754Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1091350.0134.643552
ACTGCAA1100800.0134.063351
TGCAATT1222700.0128.746923
CCGCAAT152350.0128.111772
CGCAATT160750.0126.477973
ACCGCAA155700.0125.440371
GCAATTT1640700.0109.002364
CAATTTC1668650.0107.768785
AATTTCC1677400.0107.657436
TTTCCAC1691000.0105.6341258
TTCCACA1717150.0104.253429
ATTTCCA1725250.0104.045487
CGGCAAT26200.075.4385152
ACGGCAA33650.059.618671
ATTGCAA68000.054.836121
GGCAATT38750.053.068023
ATGCAAT131300.048.7016262
TATGCAA49950.040.4545671
TCGCAAT11600.039.4646342
GCAATTC56850.038.21724
Produced by FastQC (version 0.11.5)