FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7332675
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4051115.524736879788072Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA74270.10128636548053746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT953350.0135.302642
ACTGCAA960450.0135.045881
ACCGCAA131550.0131.050611
CCGCAAT131900.0130.616522
TGCAATT1078250.0128.376023
CGCAATT142350.0127.3055653
GCAATTT1467350.0107.580724
CAATTTC1496700.0106.0245365
AATTTCC1504550.0105.884046
TTTCCAC1502850.0104.749678
TTCCACA1520400.0103.774179
ATTTCCA1531800.0103.2479557
CGGCAAT22800.082.5422442
ACGGCAA28050.067.1092151
GGCAATT38200.050.4070053
ATGCAAT120500.049.024692
ATTGCAA60000.045.909571
TCCGATC24700.043.5384675
TATGCAA47850.043.4409521
ACGCAAT15850.040.342682
Produced by FastQC (version 0.11.5)