FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5822947
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2015143.460687517849639Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA61490.10559944990054006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA786900.0135.898281
CTGCAAT794700.0134.083922
ACCGCAA111350.0133.019941
CCGCAAT112900.0130.445182
TGCAATT928850.0126.5144353
CGCAATT123850.0125.3064653
GCAATTT1260700.0106.283174
CAATTTC1282050.0104.8986055
AATTTCC1291350.0104.813556
TTTCCAC1284550.0104.053488
TTCCACA1298100.0102.905919
ATTTCCA1311350.0102.463177
CGGCAAT16800.087.7920152
ACGGCAA21800.068.0105741
ATGCAAT114200.063.9393462
ATTGCAA54550.055.5576821
ACGCAAT11700.055.2678222
GGCAATT28750.054.5863573
CATGCAA47250.049.3748741
TATGCAA41000.045.024891
Produced by FastQC (version 0.11.5)