FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4707818
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2241504.7612290874456065Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77660.16495964797279758Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT69060.14669216184652847Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT55180.11720928888924763Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT55030.1168906699451848Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT355250.0129.056692
ACTGCAA359650.0126.350431
TGCAATT398900.0124.9598853
CGCAATT42750.0113.42943
GCAATTT496700.0111.4727554
CCGCAAT40850.0110.725242
AATTTCC559950.0110.013156
CAATTTC538450.0109.212345
ACCGCAA41800.0107.0471951
TTTCCAC578700.0105.656188
ATTTCCA588050.0104.261987
TTCCACA610250.0100.365829
GACCGGA17550.069.38832
CGGCAAT13700.064.020482
AGACCGG19100.063.788491
GTTCCAC39000.057.4287645
ACCGGAA22900.054.757833
ACGGCAA16550.052.1453251
AGAACGG22500.050.9261631
ACGGAAG22350.049.942424
Produced by FastQC (version 0.11.5)